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对检测微藻营养盐限制的几种主要方法进行综述和比较,结果认为:1)细胞内各营养盐的比值法虽然比较简便,但所得值不稳定,容易受光照、CO2浓度、营养盐状况等因子影响;2)营养盐强化法是检测微藻营养盐限制最常用的一种方法,但易受微藻种类组成的影响,而且添加一种营养盐后可能导致其他营养盐的缺乏;3)营养盐吸收动力学法可应用于N、P限制的快速检测,但微藻对营养盐的吸收速率因微藻的种类、营养盐的初始浓度和营养盐状况的不同而异;4)生化法曾被广泛地用于N、P、Fe等营养盐限制的研究,但测量过程较复杂,需要时间较长,且不同种间存在差异;5)傅立叶变换红外光谱法可通过限制性营养盐添加前后微藻细胞红外光谱的变化来检测营养盐状况,能快速准确地反映检测结果,但仪器占地面积较大,且价格昂贵;6)叶绿素荧光技术具有快速、灵敏、对细胞无损伤、需要样品量少的优点,是检测微藻营养盐限制最有发展前景的方法。
The main methods for the detection of nutrient limitation in microalgae are summarized and compared. The results show that: 1) Although the ratio of nutrients in cells is relatively simple, the value obtained is not stable and easily affected by light, CO2 concentration, nutrient status And other factors; 2) Nutrient enhancement is the most commonly used method to detect nutrient limitation of microalgae, but it is susceptible to the species composition of microalgae, and the addition of one nutrient may lead to the lack of other nutrients.3 ) Nutrient absorption kinetic method can be applied to the rapid detection of N, P limits, but the absorption rate of nutrients by microalgae varies with the species of microalgae, the initial concentration of nutrients and nutrient status; 4) Biochemical France has been widely used for N, P, Fe and other nutrient limitation studies, but the measurement process is complex and takes a long time, and there are differences between different species; 5) Fourier transform infrared spectroscopy through limiting nutrients Before and after the addition of microalgae cell infrared spectroscopy changes to detect the status of nutrients, can quickly and accurately reflect the test results, but the instrument covers an area larger and more expensive; 6) Chlorophyll fluorescence technology has the advantages of fast, sensitive, , The advantage of requiring less sample is detected microalgal nutrient limitation of the most promising method.