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Objective:To identify whether Canova medication changes TNF-α and IL-10 serum levels in mice infected with Trypanosoma cruzi Y strain.Methods:Animals were divided into five groups:non-treated infected animals(I); benznidazole-treated infected animals(Bz; 100 mg/kg body weight,single daily dose by gavage); Canova medication(CM) treated infected animals(CM;0.2 mL/animal,single daily dose by gavage); benznidazole- and Canova medication–treated infected animals with the above-mentioned dose(Bz+CM);and non-infected animals(C).TNF-α and IL-10 levels were determined in serum aliquots after 4,7,10,13,and 29 days of infection.An ELISA technique was employed with R&D System Inc.antibody pairs.Results:A high increase in TNF-α and IL-10 levels occurred in the infected and CM-treated groups within the treatment employed on the 10 th day after infection,coupled with a IL-10 decrease on the 13 th day after infection when compared with the other experimental groups.Conclusions:CM may change the balance between plasma cytokine levels(TNF-α and IL-10) in mice infected with Y strain T.cruzi,with important consequences leading towards a more severe infection.
Objective: To identify whether Canova altered TNF-α and IL-10 serum levels in mice infected with Trypanosoma cruzi Y strain. Methods: Animals were divided into five groups: non-treated infected animals (I); benznidazole-treated infected animals Bz; 100 mg / kg body weight, single daily dose by gavage); Canova infected (CM; 0.2 mL / animal, single daily dose by gavage); benznidazole- and Canova- treated infected animals with the above-mentioned doses (Bz + CM); and non-infected animals (C) .TNF-α and IL-10 levels were determined in serum aliquots after 4, 7, 10, 13, and 29 days of infection. was employed with R & D System Inc. antibody pairs. Results: A high increase in TNF-α and IL-10 levels occurred in the infected and CM-treated groups within the treatment employed on the 10 th day after infection, coupled with a IL- 10 decrease on the 13 th day after infection when compared with the other experimental groups. Conclusions: CM may change the b alance between plasma cytokine levels (TNF-α and IL-10) in mice infected with Y strain T.cruzi, with important consequences leading towards a more severe infection.