目的为了解上海市金山区2009—2015年间流行性感冒流行特征,为流感防控工作提供科学依据。方法根据国家流感监测方案,采用随机抽样的原则,收集金山区流感监测点流感样病例(ILI)相关诊室以及ILI就诊情况,对部分病例进行采样开展病原学监测,使用实时荧光RT-PCR方法进行流感病毒亚型鉴定,阳性标本用MDCK细胞进行病毒分离。结果2009—2015年上海市金山区共计就诊病例2 534 969例,其中流感样病例(ILI)93 090例,占就诊人数百分比(ILI%)为3.67%,平均各周为0.01%~0.27%之间,发病集中在2~4周和28~30周;病例主要集中在5岁以下,成人病例中以25~59岁多见。检测流感样病例咽拭子标本4 625份,检出流感病毒阳性1 415株,阳性率为30.36%,其中H3型610株,B型475份,新甲型H1N1 328份,聚集性疫情中多以B型流感病毒为主,近年新甲型H1N1有上升迹象。结论该区流感发病主要集中在冬春季,以5岁以下发病为主,病原以H3和B型为主,交替出现,2009年受全球新甲型H1N1流行影响,新甲型H1N1为本地区当年优势流行株。 Objective To understand the epidemic characteristics of influenza in Jinshan District of Shanghai during 2009-2015 and provide a scientific basis for prevention and control of influenza. Methods According to the National Influenza Surveillance Program, the principle of random sampling was used to collect ILI-related clinics and ILI in Jinshan District, and some of the cases were collected for pathogen detection using real-time fluorescence RT-PCR Identification of influenza virus subtypes, positive specimens with MDCK cells for virus isolation. Results A total of 2 534 969 medical cases were collected in Jinshan District of Shanghai from 2009 to 2015. Among them, 93 090 cases of influenza-like illness (ILI) accounted for 3.67% of the total number of visits (ILI%), with an average of 0.01% -0.27% Between the incidence of 2 to 4 weeks and 28 to 30 weeks; cases are mainly concentrated in the 5-year-old, adult cases in 25 to 59 years old more common. A total of 4 625 swab samples of influenza-like cases were detected, of which 1 415 were positive for influenza virus. The positive rate was 30.36%. There were 610 strains of H3, 475 strains of B, 328 strains of new H1N1, Influenza B virus mainly in recent years, there are signs of new H1N1. Conclusion The incidence of influenza in the area mainly concentrated in the winter and spring, with the onset under 5 years old as the main pathogen, with H3 and B type as the main pathogens, alternating with the new Influenza A H1N1 epidemic in 2009, the new H1N1 Advantage of popular strains.