用冷冻麦管、细胞冷冻管分别采用一步法和二步法对Lats基因敲除小鼠胚胎进行玻璃化冷冻 ,胚胎解冻后 ,囊胚发育率分别为 90 %、90 1%、89 2 %和 91 4 % ;移植后着床率分别为 :5 0 0 %、4 7 1%、4 3 4 %和 4 6 5 % ,差异不显著 ;并且与对照组相比差异也不显著。因此 ,玻璃化冷冻可以作为Lats鼠保种方法之一 One-step and two-step methods were used to vitrify the Lats knockout mouse embryos respectively with the freezing straw and the cell freezing tube. After the embryos were thawed, the blastocyst development rates were 90%, 90 1%, 89 2% and 91 4%. The implantation rates after transplantation were respectively: 500%, 471%, 434% and 465%, with no significant difference; and the difference was not significant compared with the control group. Therefore, vitrification can be used as one of the Lats species conservation methods