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目的对奶粉中蛋白质测定时凯氏定氮法的样品前处理和分光光度法的样品前处理是否通用进行探讨。方法采用凯氏定氮法和分光光度法分别测定同一个样品前处理溶液,并对测定结果进行比对和统计学处理。结果凯氏定氮法或分光光度法测定的两种不同的样品前处理溶液,所测同一奶粉中蛋白质含量结果无统计学意义。结论凯氏法和光度法的样品前处理适用于两种测定方法通用。
Objective To determine whether the Kjeldahl method for sample preparation and spectrophotometric pretreatment of protein in milk powder are universal. Methods Kjeldahl method and spectrophotometry were used to determine the same sample pretreatment solution, and the results were compared and statistically analyzed. Results Kjeldahl method or spectrophotometric determination of the two different sample preparation solution, measured the same milk powder protein content results were not statistically significant. Conclusion Kjeldahl and spectrophotometric sample preparation is suitable for both assays.