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建立超高效液相色谱法测定食品中的γ-氨基丁酸的方法。在碱性条件下,利用邻苯二甲醛(OPA)柱前衍生法,采用Waters BEH C18分析柱(2.1 mm×50 mm×1.7μm),0.1 moL/L醋酸钾-甲醇进行洗脱,荧光检测器检测,激发波长:338 nm,发射波长:425 nm,峰面积外标法定量。γ-氨基丁酸检测浓度在0.1～20μg/mL范围内与峰面积积分值呈较好的线性关系,R=0.999 6;针对牛奶,糖果,粳米加样回收率为84.2%～110.5%,RSD=7.51(n=6),最低检测限为0.006μg/mL。本方法简便,准确,重现性好,适用于粳米,功能性添加γ-氨基丁酸的牛奶,糖果检测。
To establish a method for the determination of γ-aminobutyric acid in food by ultra performance liquid chromatography. Under alkaline conditions, the column was eluted with 0.1 mol / L potassium acetate-methanol using a Waters BEH C18 analytical column (2.1 mm × 50 mm × 1.7 μm) using OPA pre-column derivatization. Fluorescence detection Detector, excitation wavelength: 338 nm, emission wavelength: 425 nm, peak area external standard method. The concentration of γ-aminobutyric acid in the range of 0.1 ~ 20μg / mL showed a good linear relationship with the peak area integral value, R = 0.999 6; The recoveries for milk, candy and rice were 84.2% ~ 110.5% = 7.51 (n = 6), the lowest detection limit was 0.006μg / mL. The method is simple, accurate and reproducible, and is suitable for the detection of milk and candy with the function of adding γ-aminobutyric acid to rice.