论文部分内容阅读
目的:探讨虎眼万年青总皂苷诱导人肝癌HepG-2细胞凋亡的机制。方法:MTT法检测虎眼万年青总皂苷对HepG-2细胞增殖的影响;倒置显微镜观察细胞形态;流式细胞仪测定细胞凋亡率、细胞线粒体膜电位和Cyt-C蛋白表达水平;透射电镜观察细胞超微结构的改变;酶标仪检测细胞内Caspase-3活性。结果:虎眼万年青总皂苷对HepG-2细胞增殖有抑制作用,IC_(50)为(79.80±0.18)μg/m L;虎眼万年青总皂苷作用细胞后,倒置显微镜下可见细胞变圆,折光性减弱,悬浮细胞增多;透射电镜下可见细胞出现典型的凋亡特征;随着虎眼万年青总皂苷浓度的增加,细胞凋亡率、细胞内Cyt-C表达水平及Caspase-3的活性均显著升高,细胞线粒体膜电位显著降低(P<0.05或P<0.01)。结论:虎眼万年青总皂苷可显著抑制人肝癌HepG-2细胞的增殖,并可通过启动线粒体途径诱导HepG-2细胞发生凋亡。
Objective: To investigate the mechanism of the apoptosis of human hepatocellular carcinoma HepG-2 cells induced by ornithogalum total saponin. Methods: MTT assay was used to examine the effects of total saponins of Ornithogalum vulgaris on the proliferation of HepG-2 cells. The morphological changes of cells were observed under inverted microscope. The apoptosis rate, mitochondrial membrane potential and Cyt-C protein expression were measured by flow cytometry. Cell ultrastructure changes; microplate reader to detect intracellular Caspase-3 activity. Results: The total saponins of Ornithogalum vaucane inhibited the proliferation of HepG-2 cells with the IC50 of (79.80 ± 0.18) μg / m L. After treated with total saponin of Ornithogalum vulgare, the cells became round and refraction under inverted microscope The cells were characterized by transmission electron microscopy. With the increase of the concentration of total saponin of Ornithogalum, the apoptosis rate, the expression of Cyt-C and the activity of Caspase-3 were all significantly increased Increased, the mitochondrial membrane potential decreased significantly (P <0.05 or P <0.01). CONCLUSION: The total saponins from Ornithogalum can significantly inhibit the proliferation of HepG-2 cells and induce the apoptosis of HepG-2 cells by activating the mitochondrial pathway.