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目的探讨应用荧光原位杂交(FISH)技术检测胃肠道外间质瘤(EGIST)中p16基因缺失及17号染色体非整倍性的发生情况及其临床病理学意义。方法收集18例EGIST病例,采用p16/CSP17双色探针在石蜡切片上与瘤细胞杂交,所有病例同时用免疫组织化学技术检测P16蛋白的表达。结果FISH结果显示,p16基因缺失病例11例(61%),免疫组织化学检测到P16蛋白缺失病例13例(72%),基因缺失与蛋白表达差异具有统计学意义(P<0.05);共检出17号染色体非整倍体EGIST病例5例(28%)。EGIST病例中p16基因缺失、17号染色体倍体改变与各临床病理学因素间差异无统计学意义(P>0.05)。结论EGIST中存在p16基因缺失和(或)17号染色体非整倍体改变。应用FISH技术检测二者改变对于EGIST生物学行为预测具有指导意义。
Objective To investigate the occurrence of p16 gene deletion and chromosome 17 aneuploidy in gastric extraintestinal stromal tumors (EGIST) by fluorescence in situ hybridization (FISH) and its clinicopathological significance. Methods Twenty-eight EGIST cases were collected. The p16 / CSP17 two-color probe was used to hybridize with the tumor cells on the paraffin sections. In all cases, the expression of P16 protein was detected by immunohistochemistry. Results FISH showed that 11 cases (61%) had deletion of p16 gene and 13 cases (72%) had deletion of P16 protein detected by immunohistochemistry. There was a significant difference between the deletion and protein expression (P <0.05) Out of chromosome 17 aneuploidy EGIST cases in 5 cases (28%). There was no significant difference between the p16 gene and the clinicopathological factors in the EGIST cases (P> 0.05). Conclusion The deletion of p16 gene and / or aneuploidy on chromosome 17 exist in EGIST. It is instructive to detect the changes of both of them by FISH technology for the prediction of biological behavior of EGIST.