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目的探讨乌司他丁(Ulinastatin,UTI)对大鼠肝缺血-再灌注损伤的保护作用以及对白介素-18(IL-18)表达的影响。方法将大鼠随机分为3组,即假手术组、模型组、乌司他丁组。Pringle法制备大鼠肝缺血-再灌注损伤模型,检测血清中ALT、AST、SOD、MDA含量;测定肝组织中SOD、MDA水平。酶联免疫吸附法(ELISA法)测定大鼠血清及肝组织中IL-18含量。结果乌司他丁组血清中ALT、AST、MDA、IL-18水平低于模型组(P<0.05),SOD活性高于模型组(P<0.05);乌司他丁组肝组织中SOD活性高于模型组(P<0.05),MDA含量和IL-18水平低于模型组(P<0.05)。结论乌司他丁对肝缺血-再灌注损伤具有明显的保护作用,并能够干预大鼠肝缺血-再灌注对白介素-18的过度表达。
Objective To investigate the protective effect of Ulinastatin (UTI) on hepatic ischemia-reperfusion injury in rats and its effect on interleukin-18 (IL-18) expression. Methods The rats were randomly divided into three groups: sham operation group, model group and ulinastatin group. The model of hepatic ischemia-reperfusion injury was established by Pringle method. The levels of ALT, AST, SOD and MDA in serum were measured. The levels of SOD and MDA in liver tissue were determined. The contents of IL-18 in serum and liver tissue were determined by enzyme-linked immunosorbent assay (ELISA). Results The levels of ALT, AST, MDA and IL-18 in the ulinastatin group were lower than those in the untreated group (P <0.05), while the SOD activity in the ulinastatin group was higher than that in the model group (P <0.05) Higher than model group (P <0.05), MDA content and IL-18 level were lower than model group (P <0.05). Conclusion Ulinastatin has a significant protective effect on hepatic ischemia-reperfusion injury and can interfere with the over-expression of interleukin-18 induced by hepatic ischemia-reperfusion in rats.