通过酒精浸提法提取蒲公英叶中的绿原酸,采用大孔树脂DA-201分离纯化,纸片抑菌法和连续梯度稀释法研究绿原酸的抑菌效果和最小抑菌浓度。结果表明:大孔树脂对绿原酸的静态吸附量为195mg/g,动态吸附量为116.5mg/mL,最佳上样pH值为3,最佳洗脱剂为80%的酒精;通过此方法纯化的蒲公英绿原酸对大肠杆菌、枯草杆菌、根霉和青霉均有良好的抑制作用,其最小抑菌浓度分别为1×10-3、1×10-2、1×10-2、1×10-2mg/mL。 The chlorogenic acid was extracted from the leaves of Taraxacum mongolicum by ethanol extraction. The macroporous resin DA-201 was used for the isolation and purification. The antibacterial activity and the minimum inhibitory concentration of chlorogenic acid were studied by the paper antibacterial method and continuous gradient dilution method. The results showed that the macroporous resin adsorbed chlorogenic acid was 195mg / g, the dynamic adsorption capacity was 116.5mg / mL, the optimum pH value was 3, and the best eluent was 80% alcohol. The purified dandelion chlorogenic acid has a good inhibitory effect on Escherichia coli, Bacillus subtilis, Rhizopus and Penicillium, and the minimum inhibitory concentrations are 1 × 10-3, 1 × 10-2 and 1 × 10-2 , 1 × 10-2 mg / mL.