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革兰氏阴性细菌内毒素能特异性地激活鲎试剂的酶系统,被激活的酶水解显色基质(Boc-Lcu-Gly-Arg-DEAA)释放出显色基团DEAA(N,N’-二乙基苯胺),DEAA与1-萘酚-2-磺酸钠在高碘酸钠存在下生成的蓝色缩和物,在675nm波长处的吸光度与内毒素浓度在一定范围内成线性关系。1 材料和仪器1.1 1ml、5ml刻度吸管;50μl、100μl、1000μl定量移液器及对应的吸头;玻璃试管若干;旋涡混合器;恒温水浴箱;封口膜,以上均为中国药品生物制品检定所提供。日本岛津UV-2401(PC)S紫外分光光度计,玻璃器皿、塑料制品均需去除热
Gram-negative bacterial endotoxin can specifically activate the enzyme system of the limulus amebocyte lysate (Boc-Lcu-Gly-Arg-DEAA) to release the chromogenic group DEAA (N, N’- Diethylaniline), a blue condensate of DEAA and sodium 1-naphthol-2-sulfonate in the presence of sodium periodate, and the absorbance at a wavelength of 675 nm has a linear relationship with endotoxin concentration within a certain range . 1 Materials and equipment 1.1 1ml, 5ml graduated pipette; 50μl, 100μl, 1000μl quantitative pipette and the corresponding tip; a number of glass tubes; vortex mixer; constant temperature water bath; sealing membrane, the above are the China Pharmaceutical and Biological Products provide. Japan Shimadzu UV-2401 (PC) S UV spectrophotometer, glassware, plastic products are required to remove heat