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目的 观察非小细胞肺癌 (NSCLC)组织中Caspase活化的癌细胞凋亡与临床病理特征的关系。 方法 对手术切除的NSCLC组织标本 5 0例 (其中鳞癌 2 4例 ,腺癌 2 6例 ) ,采用Caspase特异的单克隆抗体—M 30CytoDEATH免疫组化染色方法显示凋亡细胞 ,计算凋亡指数 (AI)。结果 2 8.0 % ( 14 /5 0 )的肺癌组织标本中可见不同程度的M 30表达 ,其中肺鳞癌组织的M 30阳性表达率 ( 41.7% )明显高于肺腺癌 ( 15 .3% ) ,有显著性差异 (P <0 .0 5 )。肺鳞癌组织的AI亦高于腺癌 ,有显著性差异 (P <0 .0 5 )。低分化NSCLC组织的AI高于中、高分化的NSCLC组织 ,有显著性差异 (P <0 .0 5 )。低分化鳞癌组织的AI明显高于中、高分化的鳞癌 ,有显著性差异 (P <0 .0 5 )。M 30的表达与其它临床病理学特征无相关性。结论 非小细胞肺癌组织中存在Cas pase活化的自发性癌细胞凋亡机制 ,且Caspase的表达与组织类型及组织分化程度有关
Objective To observe the relationship between the apoptosis of cancer cells activated by Caspase and clinicopathological features in non-small cell lung cancer (NSCLC). Methods 50 specimens of surgically resected NSCLC specimens (including 24 squamous cell carcinomas and 26 adenocarcinomas) were used to detect apoptotic cells using a Caspase-specific monoclonal antibody-M 30CytoDEATH immunohistochemical staining method. (AI). Results There were different degrees of M 30 expression in 2 8.0 % (14 /5 0) lung cancer tissues. The positive rate of M 30 in lung squamous cell carcinoma (41.7%) was significantly higher than that in lung adenocarcinoma (15.3%). There was a significant difference (P < 0.05). The AI of lung squamous cell carcinoma tissue was also higher than that of adenocarcinoma, with a significant difference (P < 0.05). The AI of poorly differentiated NSCLC tissue was higher than that of moderately differentiated NSCLC tissue, with significant difference (P < 0.05). The AI of poorly differentiated squamous cell carcinoma tissue was significantly higher than that of moderately differentiated squamous cell carcinoma, with significant difference (P < 0.05). The expression of M 30 had no correlation with other clinicopathological features. Conclusion There is a Cas pase-activated spontaneous cancer cell apoptosis mechanism in non-small cell lung cancer, and the expression of Caspase is related to the degree of histological type and histological differentiation.