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用表达大量cDNA-编码的小鼠IL-2Rβ的大鼠转染的细胞系免疫大鼠,制备出一种抗小鼠IL-2R β链的单克隆抗体(TM-B1)。该抗体可特异性地与小鼠IL-2Rβ,cDNA 转染的细胞起反应,而不与受体细胞起反应,并可使Mr75～85kda 的小鼠IL-2Rβ产生免疫沉淀。同时表明在EL-4细胞中IL-2Rβ表达和配体的结合力两者无相互关系。TM-β1单克隆抗体可通过抑制配体与
A rat anti-mouse IL-2R β chain monoclonal antibody (TM-B1) was prepared by immunizing rats with a cell line transfected with a large number of cDNA-encoded mouse IL-2Rβ. The antibody specifically reacts with mouse IL-2Rβ, cDNA-transfected cells without reacting with recipient cells and immunoprecipitates Mr 75-85 kDa mouse IL-2Rβ. It is also shown that there is no correlation between IL-2Rβ expression and ligand binding in EL-4 cells. TM-β1 monoclonal antibodies can inhibit ligand and