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巨噬细胞是人巨细胞病毒(HCMV)的可能宿主,但HCMV在巨噬细胞中呈限制性复制,仅表达即刻早期(IE)蛋白。本文报道在经氢化可的松(HC)处理的巨噬细胞中,HCMV能进行非限制性复制,完成从IE蛋白到晚期(L)蛋白的转换,并释放感染性病毒。实验中,取HCMV阴性外周血,分离单个核细胞,经PHA-P激活的T细胞作用使巨噬细胞分化后,感染HCMVAD_(169)。并于感染后1～14天内,用空斑分析法测定其上清液中病毒效价,用免疫荧光法检测蛋白抗原。结果HC处理组病毒产量较未处理组高10～100倍,IE蛋白表达增强,其动力学与HCMV在人包皮成纤维细胞(HFF)中相似,但L蛋白表达较在HFF中迟,其产量与产病毒的细胞数相关。在未经HC处理组中,IE蛋白出现延迟24小时,L蛋白难以测出。作者指出,HC处理巨噬细胞能使HCMV复
Macrophages are likely hosts of human cytomegalovirus (HCMV), but HCMV is restricted to replicate in macrophages and expresses only the immediate early (IE) protein. Here we report that HCMV can be replicated in unlimited amounts in macrophages treated with hydrocortisone (HC), completing the conversion from IE protein to late (L) protein and release of the infectious virus. In the experiment, HCMV-negative peripheral blood was taken and mononuclear cells were isolated. Macrophages were differentiated and infected with HCMVAD_ (169) by PHA-P-activated T cells. The virus titer in supernatant was determined by plaque assay and protein antigen was detected by immunofluorescence within 1 ~ 14 days after infection. Results The virus production of HC group was 10 ~ 100 times higher than that of untreated group, and the expression of IE protein was enhanced. The kinetics of HC virus was similar to that of HCMV in human foreskin fibroblasts (HFF) And the virus-related cell number. In the HC-untreated group, the IE protein appeared delayed for 24 hours and the L protein was difficult to measure. The authors note that HC treatment of macrophages can make HCMV complex