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【目的】明确桔小实蝇Bactrocera dorsalis(Hendel)飞行肌对能源物质的利用。【方法】通过生化方法测定了能源物质代谢相关5种酶[3-磷酸甘油醛脱氢酶(GAPDH)、3-磷酸甘油脱氢酶(GDH)、乳酸脱氢酶(LDH)、柠檬酸合酶(CS)和3-羟酰辅酶A脱氢酶(HOAD)]活性的变化。【结果】桔小实蝇成虫中所测的5种酶活性随日龄的变化而变化,4日龄GAPDH,GDH,LDH和CS活性最高,20日龄HOAD活性最高。吊飞过程中,GAPDH,GDH和CS的活性变化基本一致,随吊飞时间的延长活性逐渐升高;LDH和HOAD的活性变化雌、雄虫完全不同。雄虫LDH活性除吊飞2 h外其他时间均高于静息状态,雌虫则始终低于静息状态;雄虫HOAD活性只有吊飞24 h低于静息状态水平,而雌虫吊飞后HOAD活性一直在静息状态水平及以下波动。【结论】桔小实蝇飞行所利用的能源物质包括糖类和脂肪,以糖类能源为主。吊飞过程中,雄虫除可以进行高速有氧代谢以外,还具备一定的无氧代谢能力,而雌虫只进行有氧代谢;雄虫能利用脂肪供给能量,雌虫则几乎不动用脂肪。研究结果为进一步阐明桔小实蝇的迁飞行为机制提供了依据。
【Objective】 To clarify the utilization of energy substances in muscle of Bactrocera dorsalis (Hendel). 【Method】 Five bioactive metabolites (GAPDH, GDH, LDH, citric acid) were determined by biochemical methods. (CS) and 3-hydroxyacyl-CoA dehydrogenase (HOAD)] activity. 【Result】 The results showed that the activities of 5 enzymes in adult adults of Dictyostella dorsalis changed with the changes of age. The activities of GAPDH, GDH, LDH and CS were the highest on the 4th day, and the highest on the 20th day. The activities of GAPDH, GDH and CS were basically the same during the process of hanging fly, and the activities of GAPDH, GDH and CS gradually increased with the extension of the hanging fly time. The activity changes of LDH and HOAD were completely different between female and male. The activity of LDH of male was higher than that of resting at 2 h except for 2 h, while the activity of resting LDH was still lower than that of resting at rest. The activity of HOAD of male was lower than that of resting at 24 h, while the activity of HOAD Has been at rest level and the following fluctuations. 【Conclusion】 The energy substances used by flying orange fruit fly include carbohydrates and fats, mainly carbohydrate energy. In addition to being able to perform high-speed aerobic metabolism, the male has certain oxygen-free metabolic capacity while the female only performs aerobic metabolism. The male can use the fat to supply energy and the female almost does not use the fat. The results provide the basis for further elucidation of the migration mechanism of the orange fruit fly.