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目的 观察垂体腺苷酸环化酶激活肽 (PACAP)对谷氨酸和葡萄糖缺乏所致海马神经元损伤的保护作用。方法 取新生SD大鼠海马 ,采用B2 7无血清培养基培养神经元 ,MTT法测定神经元存活率 ;分别给予谷氨酸、NMDA或葡萄糖缺乏处理 ,造成原代培养的海马神经元损伤。结果 谷氨酸、NMDA呈剂量依赖性降低海马神经元的存活率 ,葡萄糖缺乏也能明显降低海马神经元的存活率 ,而预先给予PACAP能明显增加海马神经元的存活率。结论 PACAP能保护原代培养的海马神经元 ,减轻谷氨酸和葡萄糖缺乏引起的损伤。
Objective To observe the protective effect of pituitary adenylate cyclase activating peptide (PACAP) on the damage of hippocampal neurons induced by glutamate and glucose deprivation. Methods The hippocampus of neonatal SD rats were cultured, and the neurons were cultured in B2 7 serum-free medium. The survival rate of neurons was determined by MTT assay. Glutamate, NMDA or glucose deficient groups were respectively treated, resulting in primary cultured hippocampal neurons injury. Results Glutamate and NMDA decreased the survival rate of hippocampal neurons in a dose-dependent manner. Glucose deficiency also significantly decreased the survival rate of hippocampal neurons. Pretreatment with PACAP significantly increased the survival rate of hippocampal neurons. Conclusion PACAP can protect primary cultured hippocampal neurons and reduce the damage caused by glutamate and glucose deficiency.