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目的观察通络糖泰方对自发性2型糖尿病周围神经病变大鼠Caspase-3激酶蛋白表达的影响,并探讨其作用的可能机制。方法选用GK大鼠,高脂饲料饲养造模12周,按血糖值随机分为中药高、中、低剂量组,二甲双胍和弥可保组,模型组和正常对照组。分别用不同剂量的通络糖泰浸膏、二甲双胍和弥可保、生理盐水灌胃治疗,1次/d。治疗4周后,测定各组大鼠血糖、坐骨神经病理切片、坐骨神经电生理、坐骨神经Caspase-3表达水平。结果与正常组相比,模型组大鼠坐骨神经传导速度明显减慢(P<0.01),并且组织结构出现异常,坐骨神经Caspase-3表达水平显著升高(P<0.01),治疗后,各治疗组均可下调Caspase-3激酶蛋白表达(P<0.01),以通络糖泰方中高组与中中组的效果最好(P<0.01,P<0.05)。结论通络泰方治疗DPN的可能机制之一是通过下调Caspase-3激酶蛋白表达,减少神经细胞凋亡实现对DPN进行预防与治疗。
Objective To observe the effect of Tongluo Tangtaifang on the expression of Caspase-3 kinase in spontaneous rats with type 2 diabetic peripheral neuropathy and to explore its possible mechanism. Methods GK rats were selected and fed with high-fat diet for 12 weeks. The rats were randomly divided into high, medium and low dose Chinese medicine group, metformin and miconazole group, model group and normal control group according to the blood glucose level. With different doses of Tongluo Tang Thai extract, metformin and miconazole, saline intragastric treatment, 1 / d. After 4 weeks of treatment, the blood glucose, sciatic nerve pathological sections, sciatic nerve electrophysiology and the expression of Caspase-3 in sciatic nerve of rats in each group were determined. Results Compared with the normal group, the conduction velocity of sciatic nerve in model group was significantly slowed down (P <0.01), and the structure of the model group was abnormal. The expression of Caspase-3 in sciatic nerve was significantly increased (P <0.01). After treatment, (P <0.01). The effect of Tongluo Tang Tai Fang and Zhong Zhong Tang was the best (P <0.01, P <0.05). Conclusion One of the possible mechanisms of Tongluo Taifang in treating DPN is to prevent and treat DPN by down-regulating the expression of Caspase-3 kinase and reducing the apoptosis of nerve cells.