论文部分内容阅读
目的 :使用放射线肾病动物模型 ,研究HSP4 7在放射线肾小管间质损伤中的作用。 方法 :雄性Wistar大鼠分组如下 :对照组 (n =1 2 ) ;照射组 (n =6 0 ) ,右肾切除后 ,分别给予左肾 7、1 5、2 5Gy单一剂量的放射线照射。对各组大鼠进行 9个月随访。 结果 :照射组动物早期发生肾功能不全和高血压 ,并同时产生炎症细胞浸润及肾间质纤维化。同对照组比较 ,受放射线照射的肾组织内可见HSP4 7表达增加。双重免疫染色表明 ,具有HSP4 7表达的细胞包括平滑肌肌动蛋白阳性的肌纤维母细胞 (myofibroblast)和波形蛋白 (vimentin)阳性的小管上皮细胞。而且 ,HSP4 7表达增加与肾间质内胶原沉积密切相关。 结论 :过度表达的HSP4 7通过表型变化的小管间质细胞在胶原装配、合成过程中起作用 ,并对放射线肾病小管间质纤维化的形成起着关键性作用。
OBJECTIVE: To study the role of HSP47 in radiation-induced tubulointerstitial injury using an animal model of radiation nephropathy. Methods: The male Wistar rats were divided into the following groups: control group (n = 12); irradiation group (n = 60), and right renal excision, they were given a single dose of 7,1 5,2 5 Gy single dose of the left kidney. The rats in each group were followed up for 9 months. Results: In the irradiated group, renal dysfunction and hypertension occurred early, inflammatory cell infiltration and renal interstitial fibrosis occurred at the same time. Compared with the control group, the expression of HSP47 increased in the irradiated kidneys. Double immunostaining indicated that cells with HSP47 expression included smooth muscle actin positive myofibroblast and vimentin positive tubular epithelial cells. Moreover, increased HSP47 expression is closely related to collagen deposition in the renal interstitium. Conclusion: Overexpression of HSP4 7 plays a key role in the formation of tubulointerstitial fibrosis in radiation nephropathy through the phenotypic change of tubulointerstitial cells in collagen assembly and synthesis.