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聚合酶链反应(PCR)是一种新兴的、快速的特定DNA片断体外扩增技术,目前该技术能对许多疾病的发生和预后做出准确的判定,本文通过PCR检查乙肝病毒DNA(HBV—DNA)并与放免法测定乙肝病毒标志物(HBVM)进行比较探讨两种检测方法在临床中的适用性,以提高乙肝病毒感染的诊断率,控制母婴传播、准确鉴定血制品的传染性。 1 材料与方法 1.1 标本来源:①取自传染科门诊肝功正常64例乙肝患者的血清标本;②取自住院被确诊为重
Polymerase chain reaction (PCR) is an emerging and rapid technology for in vitro amplification of specific DNA fragments. At present, this technique can accurately determine the occurrence and prognosis of many diseases. In this paper, HBV- DNA) and compared with radioimmunoassay of hepatitis B virus markers (HBVM) to explore the clinical applicability of the two detection methods in order to improve the diagnostic rate of hepatitis B virus infection, control mother to child transmission and accurately identify the infectiousness of blood products. 1 Materials and methods 1.1 The source of specimens: ① taken from the outbreak of infectious hepatitis Branch 64 cases of normal liver function in patients with hepatitis B serum samples; ② taken from the hospital was diagnosed as heavy