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ScleortiumcepivorumBerk.是引起洋葱白腐病的病原真菌,大部分测试的菌株含有dsRNA片段,这些dsRNA片段被称为真菌病毒。经琼脂糖凝胶电泳分析,在不同分离株之间或同一分离株不同提取物之间dsRNA带的数量和位置有很大的变化。ScQ-4菌株菌丝生长缓慢,致病力明显低于其他菌株。采用蔗糖密度梯度离心法证实和确定ScQ-4菌株中dsRNA片段所处的亚细胞位置,结果表明这些dsRNA片段与其寄主线粒体共同纯化,因此它们可能存在于寄主线粒体中,属于线粒体病毒。在营养相容性菌株之间这些dsRNA片段可通过菌丝融合进行传播,获得dsRNA片段的受体菌株的致病力明显降低,这对SclerotiumcepivorumBer.k进行生物防治提供了可能性。
ScleortiumcepivorumBerk. Is a pathogenic fungus that causes onion white rot. Most tested strains contain dsRNA fragments, which are called mycosis viruses. By agarose gel electrophoresis, the number and location of dsRNA bands vary greatly between different isolates or between different extracts of the same isolate. The mycelial growth of ScQ-4 strain was slow and its pathogenicity was significantly lower than that of other strains. The sucrose density gradient centrifugation was used to confirm and determine the subcellular location of the dsRNA fragments in ScQ-4. The results showed that these dsRNA fragments were co-purified with their host mitochondria. Therefore, they may exist in the host mitochondria and belong to the mitochondrial virus. These dsRNA fragments can be transmitted by mycelial fusion among the nutritionally compatible strains, and the pathogenicity of the recipient strains obtaining the dsRNA fragment is significantly reduced, which provides a possibility for the biological control of Sclerotium cepivorumBer.k.