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目的发展一种针对表皮生长因子过量表达的肿瘤细胞的基因转移系统。方法人工合成了一种Ｎ端含多个赖氨酸残基，Ｃ端为人表皮生长因子（ＥＧＦ）的受体结合域的３３肽，此合成肽（Ｋ９Ｅ肽）既具有ＤＮＡ结合活性，又能为细胞表面ＥＧＦ受体所识别并内在化。Ｋ９Ｅ肽与萤火虫荧光素酶表达质粒ｐＥＢｌｕｃ按一定比例结合形成核酸复合物，以此为基础，利用受体介导的基因转移技术，组建了外源基因转移系统。结果核酸复合物直接加入于类上皮癌细胞Ａ４３１的培养液中，４８小时后可从细胞裂解液中测出荧光素酶的显著表达。结论该系统可特异性地将外源基因导入ＥＧＦ受体过量表达的肿瘤细胞。
Objective To develop a gene transfer system targeting tumor cells overexpressing epidermal growth factor. Methods A 33-mer peptide was synthesized from the N-terminal lysine residue and the C-terminal of human epidermal growth factor receptor (EGF). The synthetic peptide (K9E peptide) has both DNA binding activity and Identified and internalized for cell surface EGF receptors. K9E peptide and firefly luciferase expression plasmid pEBluc in a certain proportion to form a nucleic acid complex, and based on this, the use of receptor-mediated gene transfer technology, the establishment of exogenous gene transfer system. Results The nucleic acid complex was directly added to the culture medium of the epithelial carcinoma A431 cells, and significant expression of luciferase was detected in the cell lysate after 48 hours. Conclusion The system can specifically introduce foreign genes into tumor cells overexpressing EGF receptor.