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目的采用高效液相色谱法测定食品中非法色素添加剂的碱性橙染料即碱性橙2(chrysoidine G,CDG)、碱性橙21(orange G,AOG)及碱性橙22(astrazon orange R,AOR)。方法样品经乙腈:水:氨水(60:40:0.5,V:V:V)在25℃下超声提取30 min,以0.02 mol/L的乙酸铵溶液(pH为5)和乙腈作为流动相进行梯度洗脱,采用Agilent XDB C_(18)色谱柱进行分离,用二极管阵列检测器在449 nm波长处检测CDG,在485 nm波长处检测AOG及AOR,以外标峰面积法定量。结果 3种碱性橙染料在0.01~0.2 mg/L浓度范围内线性关系良好,方法回收率为80.0%~94.0%,相对标准偏差均小于2%(n=6),方法的检出限为0.1 mg/kg。结论本方法操作简便准确,可适用于腐竹、干辣椒和辣椒油等不同基质样品中碱性橙的检测。
OBJECTIVE To determine the contents of chrysoidine G (CDG), orange G (AOG) and astrazon orange R (superscript 2 +), a basic orange dye for the determination of illegitimate pigment additives in food by high performance liquid chromatography AOR). Methods The samples were extracted with acetonitrile: water: ammonia (60:40:55, V: V: V) at 25 ℃ for 30 min. The mobile phase consisted of 0.02 mol / L ammonium acetate solution (pH 5) and acetonitrile Gradient elution was carried out on an Agilent XDB C_ (18) column. CDG was detected at 449 nm with a diode array detector and AOG and AOR were detected at a wavelength of 485 nm. Results The results showed that the linearity of the three basic orange dyes in the range of 0.01-0.2 mg / L was good. The recoveries were in the range of 80.0% -94.0% with relative standard deviations less than 2% (n = 6). The detection limits were 0.1 mg / kg. Conclusion The method is simple and accurate. It can be applied to the detection of alkaline orange in different matrix samples such as yuba, chili and chili oil.