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背景:已有研究证明旋转磁场干预能使大鼠骨密度显著增加,并且能维持相当长的一段时间而不下降,且与性激素无关。目的:研究旋转磁场对大鼠骨钙含量的影响,以及与血清骨碱性磷酸酶(bone-specific alkaline phosphatase,BAP)和尿脱氧吡啶交联(deoxypyridinoline crosslinks,DPD)的相关性。设计:以实验动物为对象的随机对照实验。单位:一所大学生命科学院。材料:实验于2004-03/10在深圳市微生物基因工程重点实验室完成。健康成年SD大鼠90只,雌性60只,体质量(259±70)g,雄性30只,体质量(351±104)g。按雌雄随机分9组,雌性假手术组、去卵巢常钙对照组、去卵巢低钙对照组,去卵巢低钙实验组、去卵巢常钙实验组、去卵巢低钙中药实验组、雄性对照组、雄性低钙实验组、雄性常钙实验组,每组10只。干预:雌性大鼠除假手术组以外均切除卵巢,去卵巢对照组单纯去卵巢,去卵巢实验组手术15d体内残存雌激素代谢尽后,开始旋转磁场处理15d,1次/d,2h/次。雄性大鼠除对照组外均行磁场处理15d,1次/d,2h/次。分别给予常钙(含钙0.26%的食物)、低钙(含钙0.1%的食物)和辅以中药(补骨脂、黄芪、淫羊藿、肉苁蓉免煎粉剂等)饲料饲养。旋转磁场处理后,继续饲养15d,处死后取其股骨测量各组大鼠骨钙含量,取血清测量BAP含量,取尿液测DPD含量。主要观察指标:各组大鼠骨钙含量及血清BAP和DPD变化。结果:纳入90只大鼠,实验过程中死亡4只,进入结果分析86只。①经旋转磁场处理后大鼠骨钙含量增高:去卵巢低钙中药实验组及去卵巢低钙对照组分别为(0.226±0.015),(0.206±0.015)g/g,两组比较t=4.63,P<0.05;雄性常钙实验组及雄性对照组分别为(0.206±0.031),(0.199±0.014)g/g,两组比较t=4.21,P<0.05。②经旋转磁场处理后大鼠血中BAP含量增高:去卵巢常钙实验组及去卵巢常钙对照组分别为(20.52±1.78),(15.68±3.68)U/L,两组比较t=4.76,P<0.05;雄性低钙实验组及雄性对照组分别为(17.69±3.78),(8.53±2.54)U/L,两组比较t=4.59,P<0.05。③经旋转磁场处理后实验组大鼠尿中DPD下降:去卵巢低钙中药实验组及去卵巢低钙对照组分别为(86.97±37.19),(401.57±79.34)nmol/L,两组比较t=7.45,P<0.01;雄性常钙实验组及雄性对照组分别为(97.87±31.97),(168.71±53.19)nmol/L,两组比较t=8.31,P<0.01。结论:磁场能够在短时间内(15d)有效的促进大鼠的骨钙含量增加,且增加与血BAP升高及尿DPD下降成正相关。
BACKGROUND: Previous studies have shown that the intervention of rotating magnetic field can significantly increase bone mineral density in rats, and can maintain for a long period of time without decline, and has nothing to do with sex hormones. OBJECTIVE: To study the effect of rotating magnetic field on bone calcium content in rats and its relationship with serum bone alkaline phosphatase (BAP) and deoxypyridinoline crosslinks (DPD). Design: Randomized controlled trial of experimental animals. Unit: a university life science academy. MATERIALS: Experiments were carried out in Shenzhen Key Laboratory of Microbial Genetic Engineering from March to March 2004. 90 healthy adult SD rats, 60 females, body weight (259 ± 70) g, 30 males, body weight (351 ± 104) g. The male and female were randomly divided into 9 groups: female sham operation group, normal ovariectomy control group, ovariectomized low calcium control group, ovariectomized low calcium control group, ovariectomized calcium control group, ovariectomized low calcium Chinese medicine experimental group, male control group Group, male low calcium experimental group and male calcium experimental group, 10 rats in each group. Intervention: Female rats were ovariectomized except the sham-operated group. After 15 days of ovariectomized ovariectomized ovariectomized group, the residual estrogen metabolism in the ovariectomized experimental group began to rotate 15d, 1 / d, 2h / time . Male rats except the control group were subjected to magnetic field treatment 15d, 1 time / d, 2h / times. The rats were fed with the feedstuffs of normal calcium (0.26% of calcium), low calcium (0.1% of calcium) and supplemented with traditional Chinese medicine (psoralen, astragalus, epimedium, cistanche powder, etc.) Rotating magnetic field treatment, continue feeding 15d, after sacrifice the femur to measure the bone calcium content of rats in each group, take serum BAP content, take urine test DPD content. MAIN OUTCOME MEASURES: Changes of bone calcium and serum BAP and DPD in rats in each group. RESULTS: Ninety rats were enrolled and four died during the experiment, and 86 were included in the result analysis. ①The content of calcium in rat bone after revolving magnetic field was increased: the ovariectomized low calcium group and the ovariectomized low calcium control group were (0.226 ± 0.015) and (0.206 ± 0.015) g / g respectively, t = 4.63 , P <0.05; The experimental group and the male control group were (0.206 ± 0.031), (0.199 ± 0.014) g / g, respectively, t = 4.21, P <0.05. (2) The content of BAP in the blood of the rats after rotating magnetic field treatment increased: (20.52 ± 1.78) and (15.68 ± 3.68) U / L respectively in the ovariectomized normal calcium group and the ovariectomized normal calcium control group, t = 4.76 (P <0.05). The male hypocalcemia experimental group and male control group were (17.69 ± 3.78) and (8.53 ± 2.54) U / L, respectively, t = 4.59, P <0.05. (3) The urinary DPD decreased in the experimental group after treatment with rotating magnetic field: the ovariectomized low calcium Chinese medicine group and the ovariectomized low calcium control group were (86.97 ± 37.19) and (401.57 ± 79.34) nmol / L, respectively = 7.45, P <0.01; The experimental group and the male control group were (97.87 ± 31.97) and (168.71 ± 53.19) nmol / L respectively, t = 8.31, P <0.01. CONCLUSION: Magnetic field can effectively promote the increase of bone calcium content in rats in short time (15d), and the increase is positively correlated with the increase of blood BAP and the decrease of urine DPD.