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目的:研究哺乳期2,3,7,8-TCDD暴露对大鼠牙齿的组织形态计量学的影响。方法:30d和60d龄2,3,7,8-TCDD暴露组和对照组大鼠被处死,将其带有磨牙的上颌骨行树脂包埋,制备硬组织磨片并在荧光显微镜下观察。对两实验组牙齿的组织形态、荧光标记情况及组织形态计量学参数进行比较。结果:2,3,7,8-TCDD组釉质和牙本质均相对薄弱。牙齿形态计量学参数比较,2,3,7,8-TCDD组与对照组间均显示显著性差异。牙本质计量学比较,2,3,7,8-TCDD组参数均有降低(G-Ir.L.Wi:12.58±1.54,12.58±1.54,P<0.01;MAR:1.12±0.19,1.45±0.24,P<0.01)。结论:2,3,7,8-TCDD哺乳期暴露显著降低了仔鼠牙齿的矿化程度,严重阻碍了其矿化进程。
Objective: To study the effects of 2,3,7,8-TCDD exposure during lactation on histomorphometry of rat teeth. Methods: Rats in both exposed and control groups were sacrificed at 30d and 60d after exposure to 2,3,7,8-TCDD and control group. The maxillary line with molar was embedded with resin, and hard tissue grinding discs were prepared and observed under fluorescence microscope. Tooth morphology, fluorescence labeling and histomorphometric parameters of two experimental groups were compared. Results: The enamel and dentin in the 2,3,7,8-TCDD group were relatively weak. Tooth morphometry parameters comparison, 2,3,7,8-TCDD group and control group showed significant differences. In dentin metrology, parameters of 2,3,7,8-TCDD group were decreased (G-Ir.L.Wi: 12.58 ± 1.54,12.58 ± 1.54, P <0.01; MAR: 1.12 ± 0.19,1.45 ± 0.24 , P <0.01). Conclusion: Exposure to 2,3,7,8-TCDD during lactation significantly reduced the mineralization of the teeth of the offspring and severely hindered the mineralization process.