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目的研究博莱霉素(BLM)所致大鼠肺纤维化的病理形态学变化和三七总皂甙(PNS)的干预作用及其机制。方法通过气管内注入BLM复制动物肺纤维化模型,于造模后第2天各治疗组开始每天给药,于给药后第7,14,28天分别随机处死一批大鼠,取肺组织行HE染色,用免疫组织化学方法检测肺组织中TGF-β1的表达,并测定肺组织中羟脯氨酸(HYP)含量。结果PNS能抑制实验性肺纤维化大鼠肺组织中胶原沉积及TGF-β1的异常升高,减轻其肺部的病理损害。结论PNS对BLM诱导产生的肺纤维化有一定的抑制作用。
Objective To investigate the pathological changes of pulmonary fibrosis induced by bleomycin (BLM) in rats and the intervention effect of Panax Notoginseng Saponins (PNS) and its mechanism. Methods Pulmonary fibrosis model was established by intratracheal instillation of BLM. On the second day after model establishment, each treatment group began daily administration. A group of rats were randomly sacrificed on the 7th, 14th and 28th days after the administration, and lung tissues The expression of TGF-β1 in lung tissue was detected by immunohistochemistry and the content of hydroxyproline (HYP) in lung tissue was determined by HE staining. Results PNS could inhibit collagen deposition and abnormal TGF-β1 in the lungs of experimental pulmonary fibrosis rats and alleviate the pathological damage of the lungs. Conclusion PNS can inhibit pulmonary fibrosis induced by BLM to a certain extent.