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目的:了解环一磷酸腺苷(cAMP)、环一磷酸鸟苷(cGMP)含量的变化与脑缺血、再灌注损伤和功能恢复间的关系。方法:以大鼠四血管阻断为模型,采用放免法测定不同状态下血浆和皮层、海马、纹状体中cAMP、cGMP的含量。结果:cAMP在各脑区和血浆中的含量,缺血组、再灌组、腺苷和N6环乙烷基腺苷(CHA)给药组分别与对照组相比均无显著性变化(P>005)。cGMP的血浆含量由对照组的532±135pmol/ml增高至再灌组的1846±417pmol/ml(P<001)。给药3、4组也分别增高至2015±562pmol/ml和2265±834pmol/ml,(P<001),但与再灌组相比无显著性差异(P>005)。其余血浆各组及脑区各组cGMP含量均无显著性变化。结论:各脑区内cAMP和cGMP含量与大鼠脑缺血损伤和恢复无直接关系。血浆内cAMP含量无显著性变化,cGMP含量虽有变化,但作为临床观测指标证据不足。
Objective: To investigate the relationship between changes of cAMP and cGMP, cerebral ischemia, reperfusion injury and functional recovery. Methods: Rat model of four-vessel occlusion was used to determine the contents of cAMP and cGMP in plasma and cortex, hippocampus and striatum by radioimmunoassay. Results: The content of cAMP in the brain regions and plasma did not change significantly compared with the control group in the ischemic group, reperfusion group, adenosine group and N6cycloalkyladenosine (CHA) administration group P> 005). Plasma levels of cGMP increased from 532 ± 1 35 pmol / ml in the control group to 18 46 +4 17 pmol / ml in the reperfusion group (P <0 01). 3,4 and 4 groups were also increased to 20 15 ± 5 62 pmol / ml and 22 65 ± 8 34 pmol / ml, (P <0 01), but compared with the reperfusion group was no significant difference ( P> 005). There was no significant change in cGMP content in other plasma groups and brain regions. Conclusion: There is no direct relationship between cAMP and cGMP content in brain regions and cerebral ischemia injury and recovery in rats. There was no significant change in plasma cAMP content, cGMP content despite changes, but as evidence of lack of clinical evidence.