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目的:探讨环氧合酶-2(COX-2)和血管内皮生长因子(VEGF)在子宫内膜异位症(EMs)组织中的表达,以及COX-2及VEGF对血管形成的影响。为子宫内膜异位症的发病机制、治疗以及预防的研究提供理论依据。方法:应用链霉素抗生物素蛋白-过氧化物酶连接免疫组织化学法对32例子宫内膜异位症患者组织中COX-2和VEGF的表达进行检测。结果:环氧合酶-2在正常子宫内膜(54.50%)、子宫内膜异位症在位内膜(62.50%)及子宫内膜异位症异位组织中(81.25%)的表达阳性率逐渐升高,差异有显著性意义(P<0.01);正常内膜及子宫内膜异位症在位内膜中环氧合酶-2表达受月经周期影响,分泌期的阳性表达率(60.00%,66.67%)高于增生期(50.00%,57.14%),差异有显著性意义(P<0.05,p<0.01);血管内皮生长因子在正常内膜(51.51%)、子宫内膜异位症在位内膜(59.38%)及子宫内膜异位症异位组织中(93.75%)的表达阳性率逐渐升高,差异有显著性意义(P<0.01)。正常内膜及子宫内膜异位症在位内膜中血管内皮生长因子表达受月经周期影响,分泌期的阳性表达率(53.33%,72.22%)高于增生期(50.00%,42.86%),差异有显著性意义(P<0.01),在子宫内膜异位症中环氧合酶-2、血管内皮生长因子二者密切相关(r=0.78)。结论:COX-2和VEGF在EMs组织中存在高表达,COX-2可能通过增加VEGF表达而促进EMs的血管形成,二者在血管生成过程中密切相关,从而在EMs发生、发展中起关键的作用。
Objective: To investigate the expression of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) in endometriosis (EMs) tissues and the effect of COX-2 and VEGF on angiogenesis. It provides a theoretical basis for the study of the pathogenesis, treatment and prevention of endometriosis. Methods: The expression of COX-2 and VEGF in tissues of 32 patients with endometriosis was detected by streptavidin-peroxidase-linked immunohistochemistry. Results: The expression of cyclooxygenase-2 in normal endometrium (54.50%), endometriosis (62.50%) and ectopic endometriosis (81.25%) were positive (P <0.01). The expression of cyclooxygenase-2 in eutopic and eutopic endometrium of normal endometrium and endometriosis was affected by menstrual cycle and the positive rate of secretory phase was 60.00% and 66.67% respectively) were higher than those in proliferative stage (50.00%, 57.14%) (P <0.05, p <0.01). The expression of vascular endothelial growth factor in normal endometrium (51.51% The positive expression rate of eutopic endometrium (59.38%) and ectopic endometriosis (93.75%) were gradually increased, the difference was significant (P <0.01). The expression of VEGF in eutopic and eutopic endometrium of normal endometrium and endometriosis was affected by the menstrual cycle. The positive expression rates in the secretory phase (53.33%, 72.22%) were higher than those in the proliferative phase (50.00%, 42.86%), (P <0.01). There was a close correlation between cyclooxygenase-2 and vascular endothelial growth factor in endometriosis (r = 0.78). CONCLUSIONS: COX-2 and VEGF are highly expressed in EMs. COX-2 may promote angiogenesis of EMs by increasing VEGF expression, which is closely related to the process of angiogenesis and thus play a key role in the development and progression of EMs effect.