获得银耳菌种的方法主要有担孢子弹射分离法、组织分离法和耳木分离法。1孢子弹射法这是利用新鲜的、成熟的子实体自动弹射出担孢子来获得纯菌种的方法。取朵大、色白、无病虫害的新鲜银耳作为种耳。用无菌水漂洗银耳数次,再用无菌纱布或吸水纸,把银耳表面的水分吸干。因为耳片表面若有薄水层,便会影响银耳担孢子的弹射,并容易污染上杂菌。再把种耳切一小块,以放入三角瓶或放入试管中不会碰到瓶壁或管壁为度,随后用不锈钢小钩钩住小耳片,迅速悬挂在三角瓶或试管内,同时注意勿使种耳碰到培养基表面,以防污染杂菌,塞上棉塞,然后放在20℃~25℃条件下,经过24 h,从耳片上弹射出来的担孢子,落在光滑的培养基表面上形成1个雾状的孢子印。此时,在无菌箱或无菌室 The main methods to obtain fungus species are basidiospora ejection separation, tissue separation and ear separation method. 1 spore projectile method This is the use of fresh, mature fruiting bodies automatically shoot out spores to obtain pure species method. Take big, white, pest-free fresh white fungus as the ear. White fungus rinse several times with sterile water, and then sterile gauze or absorbent paper, the surface of the white fungus dry. Because the surface of the ear, if there is a thin layer of water, will affect the eruption of the fungus spores, and easily contaminated bacteria. Then cut a small piece of ear to put into a triangular flask or into the test tube will not touch the bottle wall or wall for the degree, then hook the small stainless steel hook ear, quickly hanging in a triangular flask or tube, at the same time Be careful not to hit the ear to the media surface to prevent contamination of bacteria, tampon stuffed, and then placed in the 20 ℃ ~ 25 ℃ conditions, after 24 h, from the ear prolactin spores, fell on a smooth One mist-like spore seal formed on the surface of the medium. At this point, in a sterile box or sterile room