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目的探讨小脑延髓池注射纳洛酮对心肺复苏大鼠脑神经保护的作用机制。方法将30只雄性SD大鼠随机分为假手术组、常规复苏组和纳洛酮复苏组。采用窒息法建立大鼠心脏骤停模型,复苏的同时给予药物治疗。恢复自主循环(ROSC)后24 h取脑组织,荧光定量PCR法检测脑组织c-Fos mRNA表达水平,免疫组化法检测脑组织c-Fos蛋白的表达。结果与常规复苏组比较,纳洛酮可显著降低大鼠脑组织c-Fos mRNA及蛋白表达量(P<0.01)。结论小脑延髓池注射纳洛酮可及时有效的作用于c-Fos基因,发挥脑神经保护作用。
Objective To investigate the effect of naloxone injected into the cisterna magna on cerebral neuroprotection in rats with cardiopulmonary resuscitation. Methods Thirty male Sprague-Dawley rats were randomly divided into sham-operation group, routine resuscitation group and naloxone-resuscitation group. The asphyxia method was used to establish a cardiac arrest model in rats, while resuscitation was given drug treatment. The brain tissues were harvested 24 hours after the recovery of spontaneous circulation (ROSC). The expression of c-Fos mRNA was detected by real-time quantitative PCR. The expression of c-Fos protein was detected by immunohistochemistry. Results Compared with the conventional resuscitation group, naloxone significantly decreased the expression of c-Fos mRNA and protein in the rat brain (P <0.01). Conclusion Injection of naloxone into the cerebellar cisterna cisterna can act on c-Fos gene in time and effectively and exert the neuroprotective effects.