原位ＰＣＲ防脱片方法的选择及应用肖莎朱新生郭琳琅原位ＰＣＲ是ＰＣＲ与原位杂交两种方法的结合，其最大的特点就是将附在载玻片上组织切片内的靶基因原位扩增、原位检测、原位观察。这对于探明靶基因与病变发生发展的关系十分重要。然而原位ＰＣＲ步骤多，时间长，其间... Selection and application of in situ PCR anti-shedding method Xiao Sha Zhu Xinsheng Guo Linlang In situ PCR is a combination of PCR and in situ hybridization. The most important feature of this method is that the target gene Amplification, in situ detection, in situ observation. This is very important for determining the relationship between the target gene and the development of the lesion. However, more steps in situ PCR, a long time, during which ...