目的　通过DNA序列分析 ,确定辽宁省HIV -1流行毒株的亚型。方法　从辽宁省HIV -1抗体阳性者中选择 16例 ,其中经性接触感染者 8例 ,经静脉吸毒和血液途径感染者 7例 ,垂直传播者 1例 ,采集全血提取DNA作为PCR扩增的模板 ,套式引物扩增HIV -1前病毒DNA的C2～V 3区用于测序。所测序列与国际标准株及国内外参考序列进行比较 ,确定被检标本的亚型。结果　辽宁省流行的HIV -1病毒分属A、B、C、G4种亚型 ,其中经性途径传播者 8例分别为B′亚型和A亚型 ,静脉吸毒者 4皆为C亚型 ,经血途径感染者分别属B′和G亚型 ,垂直传播者为A亚型。亚型内基因离散率分别为 :A亚型 (7 5± 1 0 ) % ,B′亚型 (8 5± 0 9) % ,C亚型 (5 4± 0 9) % ,G亚型 (13 5±2 1) %。结论　辽宁省目前HIV -1的流行株亚型较为复杂 ,不同传播途径感染HIV毒株亚型有所不同 ,C亚型毒株只见于静脉吸毒者 ,经性途径和血途径感染主要为B′亚型 ,而A亚型和G亚型只见于非洲劳务输出人员及其配偶子女。 Objective To determine the subtypes of HIV-1 epidemic strains in Liaoning Province by DNA sequence analysis. Methods Sixteen cases were selected from HIV-1 positive individuals in Liaoning Province. Among them, 8 were infected by sexual contact, 7 were infected by intravenous drug and blood, 7 were vertical transmission, and DNA was extracted from whole blood for PCR amplification The set of primers was used to amplify C2 ~ V3 regions of HIV-1 proviral DNA for sequencing. The tested sequences are compared with the international standard strains and the reference sequences at home and abroad to determine the subtype of the test sample. Results The prevalence of HIV-1 virus in Liaoning province belonged to A, B, C and G subtypes. Among them, 8 cases were sexually transmitted by B and A subtypes, while those with IV drug use were all C subtype , Menstrual pathogen infection were B ’and G subtype, vertical transmission is A subtype. The subgenotype genetic divergence rate was 75.5% in subtype A, 85.5% in subtype B, 84.5% in subtype C, 13 5 ± 2 1)%. Conclusions The prevalence of HIV-1 strains in Liaoning Province is quite complicated at present. The subtypes of HIV-infected strains in different transmission routes are different. The C-subtype strains are found only in intravenous drug users. The main pathogenic infections are B ’ Subtypes, while subtypes A and G are found only in Africa’s labor exporters and their spouse’s children.