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目的 :研究阉割和睾酮替代对束缚应激模型大鼠血清中应激免疫抑制蛋白的影响。方法 :成年雄性SD大鼠随机分为正常对照组、假手术组、阉割组、阉割后睾酮替代组。每组 10～ 12只。以上各组随机抽出一半给予束缚应激 ,另一半作为不应激对照。观察各组大鼠血清对ConA诱导的淋巴细胞转化的作用 ,作为反映血清中应激免疫抑制蛋白含量的指标。在离体实验中观察不同浓度的睾酮对淋巴细胞转化的直接作用。结果 :阉割可促进应激大鼠血清中应激免疫抑制蛋白的产生 ,睾酮替代可对抗这一作用。结论 :睾酮可在一定范围内对抗应激免疫抑制蛋白的产生。
Objective: To study the effects of castration and testosterone replacement on the expression of stress immunosuppressive proteins in the serum of restraint stress rats. Methods: Adult male Sprague-Dawley rats were randomly divided into normal control group, sham operation group, castration group and post castration testosterone replacement group. Each group of 10 to 12. More than half of the above groups randomly assigned to restraint stress, the other half as stress control. The effect of serum of each group on ConA-induced lymphocyte transformation was observed as an index to reflect the content of stress immunosuppressive protein in serum. In vitro experiments to observe the different concentrations of testosterone on the direct role of lymphocyte transformation. Results: Castration can promote the production of stress immunosuppressive proteins in the serum of stressed rats. Testosterone replacement can counteract this effect. Conclusion: Testosterone can antagonize the production of stress immunosuppressive proteins in a certain range.