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目的:定量分析喉鳞状细胞癌(LSCC)癌细胞核的形态,并探讨形态学参数与肿瘤临床的关系。方法:采用全自动细胞图像分析仪对来源于21例LSCC和20例喉部良性病变的83张细胞刷片做核形态定量研究。结果:LSCC癌细胞表现为较大的核面积、平均半径、核周长、核圆球度以及核单位面积DNA含量,其中核面积、核周长、核单位面积DNA含量在肿瘤发生不同部位及不同病理类型间有差异,喉部恶性肿瘤组织中S期细胞细胞核均在77μm2以上,平均核半径和核面积作拟合抛物线(拟合方程Y=aX2+bX+c),提示b值越小(b<0.045),c值越大(c>1.82),则与肿瘤越相关。结论:核形态定量分析认为LSCC癌细胞表现为异常的核形态,结合S期细胞核面积与平均半径和核面积的抛物线拟合方程系数,在辅助细胞学诊断及临床评价肿瘤生物学特性方面意义较大。
Objective: To quantitatively analyze the nuclear morphology of laryngeal squamous cell carcinoma (LSCC) and to explore the relationship between morphological parameters and tumor clinical. METHODS: Totally 83 cell lines from 21 cases of LSCC and 20 cases of benign laryngeal lesions were examined for their nuclear morphology using an automated cell image analyzer. Results: The LSCC cells showed larger nuclear area, average radius, nuclear perimeter, nucleus sphericity and nuclear DNA content per unit area. The nuclear area, perinuclear area and nuclear DNA content in different parts of the tumor and There were differences among different pathological types. The cell nuclei of S phase in malignant laryngeal tissues were above 77μm2, and the average nuclear radius and nucleus area were fitted to a parabola (fitting equation Y = aX2 + bX + c), suggesting that the smaller the b value (b <0.045), the greater the c value (c> 1.82), the more relevant to the tumor. Conclusion: Quantitative nuclear morphological analysis shows that the LSCC cancer cells exhibit abnormal nuclear morphology. Combined with the coefficient of parabola fitting equation of nucleus area and average radius and nucleus area of S phase, the significance of cytological diagnosis and clinical evaluation of tumor biological characteristics Big.