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目的应用同种异体软骨细胞移植修复兔膝关节软骨缺损,评估术后免疫学表现及其修复效果。方法供体为1月龄新西兰兔1只,取其膝关节全层软骨片,0.25%胰蛋白酶和0.2%Ⅱ型胶原酶消化,分离培养软骨细胞。受体为4月龄新西兰兔30只,暴露股骨髁前面关节面,用钻在股骨内髁制作直径4mm、深2mm(达髓腔)的关节软骨缺损模型。软骨细胞移植组植入体外培养12d的PLGA软骨细胞复合体,PLGA移植组只植入PLGA,假手术组只做皮肤切开手术。术后不固定患肢,兔笼中活动。术前、术后3、5、7、12周分离受体外周血淋巴细胞,检测其与异体软骨细胞和PLGA混合后的刺激指数(stimulation index,SI);术后5、7、12周取修复区软骨及软骨下骨观察局部组织学反应。结果软骨细胞移植组术后检测到淋巴细胞增殖,移植物局部见到淋巴细胞浸润。结论同种异体软骨细胞复合PLGA移植存在免疫反应。
Objective To repair allograft cartilage defects in rabbits by allogeneic chondrocyte transplantation and evaluate the postoperative immunological performance and its repair effect. Methods The donor was a New Zealand rabbit aged 1 month. Chondrocytes were isolated and cultured by digesting the whole knee cartilage with 0.25% trypsin and 0.2% collagenase Ⅱ. Recipients were 30 New Zealand rabbits aged 4 months. The anterior articular surface of the femoral condyle was exposed and a 4 mm in diameter and 2 mm deep (medullary cavity) articular cartilage defect model was made by drilling the medial condyle of the femur. The chondrocyte transplantation group was implanted into the PLGA chondrocyte complex cultured in vitro for 12 days. Only PLGA was implanted in the PLGA transplantation group, and the sham operation group was only performed skin incision. Postoperative non-fixed limbs, rabbit cage activities. Peripheral blood lymphocytes were isolated preoperatively and at 3, 5, 7, 12 weeks after operation, and the stimulation indices (SI) were measured after mixed with allogenic chondrocytes and PLGA. Repair regional cartilage and subchondral bone to observe the local histological reaction. Results Lymphocyte proliferation was detected in the chondrocyte transplantation group and lymphocyte infiltration was seen locally in the graft. Conclusion Allogeneic chondrocytes have immunologic reaction in PLGA transplantation.