论文部分内容阅读
目的 观察己酮可可碱 (PTX)对呼吸道合胞病毒 (RSV)所诱导的人肺泡灌洗液巨噬细胞肿瘤坏死因子 (TNF α)含量及TNF αmRNA表达水平的影响。方法 收集咳嗽患者正常镜像的支气管肺泡灌洗液巨噬细胞 ,随机分成三组 :(1)对照组 (NOR组 ) ;(2 )感染组 (RSV组 )加入10 6pfu的RSV ;(3 )己酮可可碱组 (PTX组 )RSV感染后加入PTX (1mg/ml)。每组于RSV感染2 0h后 ,用ELISA法测定培养细胞上清中TNF α含量的变化 ,用逆转录聚合酶链反应 (RT PCR)测定各组巨噬细胞TNF αmRNA表达水平。结果 感染组中TNF αmRNA表达水平与对照组相比明显增多。PTX组TNF αmRNA的表达和感染组相比明显降低。TNF α的含量也有相应的变化 :与对照组比较 ,感染组明显升高 (P <0 .0 1) ;而PTX组与感染组比较明显下降 (P <0 .0 1)。结论 PTX能抑制RSV所诱导的人肺泡巨噬细胞TNF α的基因表达 ,并减少TNF α的产生。
Objective To investigate the effects of pentoxifylline (PTX) on the levels of tumor necrosis factor α (TNF α) and TNF α mRNA in human bronchoalveolar lavage fluid (BALF) induced by respiratory syncytial virus (RSV). Methods Normal bronchoalveolar lavage fluid macrophages from patients with cough were collected and randomly divided into three groups: (1) control group (NOR group); (2) RSV group; 10 6 pfu of RSV; (3) PTX (1 mg / ml) was added after exposure to ketamine in the PTX group. Twenty hours after RSV infection, the changes of TNFα in supernatant of culture supernatant were detected by ELISA. The expression of TNFα mRNA of macrophages in each group was determined by reverse transcriptase-polymerase chain reaction (RT PCR). Results The level of TNFαmRNA in infected group was significantly higher than that in control group. The expression of TNFα mRNA in PTX group was significantly lower than that in the infected group. The content of TNFα also changed accordingly: Compared with the control group, the infection group was significantly increased (P <0.01), while the PTX group and the infection group was significantly decreased (P <0.01). Conclusion PTX can inhibit the gene expression of TNFα induced by RSV in human alveolar macrophages and decrease the production of TNFα.