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目的 :了解抗氧化酶和抗氧化物之间的关系及其在老年性白内障形成中的作用。方法 :随机取符合条件的老年性白内障 44例(44眼 ) ,男 2 3例 ,女 2 1例 ,平均年龄 6 7.7岁。用黄嘌呤氧化酶法测定超氧化物歧化酶 (Superoxide dismutase,SOD)、丙二醛(Malondialdehyde MDA)用巴比妥酸反应比色法测定 ,谷胱甘肽过氧化物酶 (Glutathione- Peroxide,GSH- PX)用改进的还原型谷胱甘肽消耗法 ,Vit C用微量荧光法测定 ,Vit E用比色法测定。结果 :SOD活性在血、房水、晶体核中的活性后二者低于血中 ,但无统计学差异 (F—检验 ,P>0 .0 5 ) ;GSH— PX、 Vit C、 Vit E在血—房水和血—晶体核中的变化有显著性差异 (F—检验 ,P<0 .0 1) ;MDA在血—房水、房水—晶体核中的含量有显著性差异 (F—检验 ,P<0 .0 1)。结论 :老年性白内障的形成可能是由于房水和晶体核中抗氧化酶活性下降及抗氧化物减少和抗氧化酶之间和抗氧化物之间以及二者之间相互协调、制约关系受到部分破坏所致
Objective: To understand the relationship between antioxidant enzymes and antioxidants and their role in the formation of senile cataract. Methods: Forty-four patients (44 eyes) with senile cataract were selected randomly. There were 23 males and 21 females, with a mean age of 6 7.7 years. The levels of superoxide dismutase (SOD), malondialdehyde MDA (MDA) were measured by xanthine oxidase method and the activity of glutathione peroxidase (Glutathione-Peroxide, GSH-PX) with an improved reduced glutathione depletion method, Vit C with a micro-fluorescence method, and Vit E with a colorimetric method. Results: There was no significant difference in the activity of SOD between blood and aqueous humor and crystal nucleus (F-test, P> 0.05); GSH- PX, Vit C and Vit E There was significant difference in the blood-aqueous and blood-crystal nuclei (F-test, P <0.01); the contents of MDA in blood-aqueous humor and aqueous humor-crystal nucleus were significantly different F-test, P <0.01). Conclusions: The formation of senile cataract may be due to aqueous humor and crystal nucleus in the decline of antioxidant enzyme activity and antioxidants decreased and antioxidant enzymes and antioxidants, as well as between the two coordinated by the relationship between the part by Damage caused