苏州医学院附二院神经外补脑肿瘤研究室黄强等（215004）从该室先前建立的鼠原性抗人脑胶质瘤单抗SZs。杂交瘤细胞中抽提总RNA，RTPCR扩增VH和VL基因，测序结果分别为348hP（116个氨基酸）和318bp（106个氨基酸），证实为SZ39。重、轻键可变区基因后，采用重组技术，分别将VH和 Wuqiang et al. (215004) from the Second Affiliated Hospital of Suzhou Medical College, previously established the mouse original anti-human glioma monoclonal antibody SZs from this laboratory. Total RNA was extracted from the hybridoma cells and the VH and VL genes were amplified by RTPCR. The sequencing results were 348 hP (116 amino acids) and 318 bp (106 amino acids), respectively, confirming that it was SZ39. After the heavy and light keys of the variable region genes, recombinant techniques were used to separate the VH and