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目的观察不同浓度糖基化终末产物(Advanced glycation end products,AGEs)及AGEs作用不同时间对大鼠肾小球系膜细胞尾加压素Ⅱ(UrotensinⅡ,UⅡ)及G蛋白偶联受体(G-protein-couple receptor,GPR14)mRNA表达的影响。方法制备AGE-BSA,体外培养大鼠肾小球系膜细胞(Mesangial Cells,MC),加入不同浓度的AGE-BSA(终浓度分别为0、25、50、100和200mg/L),37℃孵育24h;加入100mg/L AGE-BSA,分别培养0、2、8、16和24h,以不含葡萄糖的BSA作为对照。收集细胞,采用RT-PCR检测各组MC UⅡ及GPR14mRNA的表达。结果 AGE-BSA各组MC UⅡ及GPR14mRNA的表达量均随AGEs浓度的增加而增加,50、100和200mg/L与0mg/L组比较,差异有统计学意义(P<0.05);100mg/L AGE-BSA各组MC UⅡ及GPR14mRNA的表达量随着作用时间的延长而增加,作用8、16、24h组与0h组比较,差异有统计学意义(P<0.05)。BSA组MC UⅡ及GPR14mRNA的表达量无明显增加(P>0.05)。结论 AGEs能上调大鼠MC UⅡ及GPR14mRNA的表达。
Objective To observe the effect of different concentrations of advanced glycation end products (AGEs) and AGEs on the expressions of urotensin Ⅱ, UⅡ and G-protein coupled receptors G-protein-couple receptor, GPR14) mRNA expression. Methods AGE-BSA was cultured in vitro. Mesangial cells (MC) were cultured in vitro. AGE-BSA (0, 25, 50, 100 and 200 mg / L, The cells were incubated with 100 mg / L AGE-BSA for 0, 2, 8, 16 and 24 h, respectively. Glucose-free BSA was used as a control. The cells were collected and the expression of MC UⅡ and GPR14 mRNA in each group was detected by RT-PCR. Results The expression of MC UⅡ and GPR14 mRNA in AGE-BSA groups increased with the increase of AGEs concentration. There were significant differences between 50, 100 and 200 mg / L groups and 0 mg / L groups (P <0.05) The expression of MC UⅡ and GPR14 mRNA in AGE-BSA groups increased with the prolongation of time, and the difference was statistically significant (P <0.05). The expression of MC UⅡ and GPR14 mRNA in BSA group had no significant increase (P> 0.05). Conclusion AGEs can up-regulate the expression of MC UⅡ and GPR14 mRNA in rats.