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目的评价椰果的安全性,为微生物纤维素食品的开发提供依据。方法椰果脱水制成干粉后按GB15193-2003规定的方法进行小鼠急性经口毒性试验、遗传毒性试验(Ames试验、小鼠骨髓微核试验、小鼠精子畸形试验)、30d喂养试验及致畸试验。结果小鼠急性经口毒性MTD大于15g/kg·bw,三项遗传毒性试验结果均为阴性,30d喂养试验大鼠的生长发育、脏体比、组织病理学、血液和生化指标无异常变化,最大无损害作用剂量估算大于5.0 g/kg·bw,为人体推荐摄入量的100倍,致畸试验对孕鼠无明显的母体毒性,对胎鼠亦无明显胚胎毒性和致畸性。结论椰果干粉属无毒级和无遗传毒性物,在本实验剂量范围内属安全受试物,验证了椰果的食品安全性。
Objective To evaluate the safety of coconut and provide the basis for the development of microbial cellulose food. Methods Coconut dewatered into dry powder and then subjected to acute oral toxicity test, genotoxicity test (Ames test, mouse bone marrow micronucleus test, mouse sperm abnormality test), and 30d feeding test according to the method specified in GB15193-2003 Abnormal test. Results The acute oral toxicity of MTD in mice was more than 15g / kg · bw. The results of three genotoxicity tests were negative. The growth and development, the ratio of visceral body, histopathology, blood and biochemical indexes of 30d-fed rats showed no abnormal changes, The maximum non-damaging dose is estimated to be more than 5.0 g / kg · bw, which is 100 times of the recommended intake. The teratogenicity test shows no obvious maternal toxicity to pregnant rats and no embryotoxicity and teratogenicity to fetus. Conclusion Coconut dry powder is a non-toxic grade and no genotoxic substances, in the experimental dose range of safe subjects, to verify the safety of coconut food.