论文部分内容阅读
目的 探讨缺氧诱导因子 -2α(Hypoxiainduciblefactor -2α ,HIF -2α)、血管内皮生长因子 (vascularendothelialgrowthfac tor ,VEGF)在肾细胞癌中的表达及其与血管生成、癌转移的关系。方法 应用免疫组织化学技术检测 48例肾细胞癌中HIF -2α、VEGF的表达。结果 48例肾细胞癌HIF -2α阳性率为 70 8%,强表达 12例 ,中度表达 10例 ,弱表达 12例 ,无表达 14例 ,与正常对照组比较有显著性差异 (P <0 0 1)。Ⅰ级、Ⅱ级、Ⅲ～Ⅳ级VEGF阳性细胞数三组比较有显著性差异 (P <0 0 1)。患者癌转移者与无癌转移者HIF -2α、VEGF比较有显著性差异 ( P <0 0 1) ,HIF -2α表达与肾细胞癌恶性程度呈正相关 (r =0 682 ,P <0 0 1)。VEGF与HIF -2α表达呈正相关 (r =0 72 2 ,P <0 0 1)。结论 HIF -2α与VEGF有相关性 ,HIF -2α、VEGF与血管生成、癌转移有关 ,HIF -2α与VEGF联合检测有助于判断肾细胞癌患者的预后
Objective To investigate the expression of hypoxia inducible factor-2α (HIF-2α) and vascular endothelial growth factor (VEGF) in renal cell carcinoma and its relationship with angiogenesis and metastasis. Methods Immunohistochemistry was used to detect the expression of HIF - 2α and VEGF in 48 cases of renal cell carcinoma. Results The positive rate of HIF - 2α in 48 cases of renal cell carcinoma was 70.8%, strong expression was 12 cases, moderate expression was 10 cases, weak expression was 12 cases and no expression was 14 cases, which was significantly different from the normal control group (P <0 0 1). The number of VEGF-positive cells of grade Ⅰ, Ⅱ and Ⅲ ~ Ⅳ were significantly different among the three groups (P <0.01). There was a significant difference between HIF-2α and VEGF in patients with cancer metastasis and those without metastasis (P <0.01). HIF-2α expression was positively correlated with malignancy of renal cell carcinoma (r = 0 682, P 0 01 ). There was a positive correlation between VEGF and HIF-2α expression (r = 0 72 2, P <0.01). Conclusions There is a correlation between HIF-2α and VEGF. HIF-2α and VEGF are associated with angiogenesis and metastasis. Combined detection of HIF-2α and VEGF may be helpful in judging the prognosis of patients with renal cell carcinoma