目的探讨右旋糖酐-磁性层状复合氢氧化物-氟尿嘧啶给药系统对体外培养结肠癌SW480细胞增殖的抑制效果与诱导凋亡作用。方法采用MTT法检测各浓度右旋糖酐-磁性层状复合氢氧化物-氟尿嘧啶及所涉及各级超分子对结肠癌细胞的增殖抑制率;光镜显微、Giemsa染色表征细胞的形态变化;流式细胞术检测药物干预引起的SW480细胞凋亡率;DNA ladder分析凋亡细胞核的裂解情况。结果磁性层状复合氢氧化物、磁性层状复合氢氧化物-氟尿嘧啶、右旋糖酐-磁性层状复合氢氧化物-氟尿嘧啶3级超分子对SW480细胞干预24 h的IC50依次为44.83(25.74,48.78)μg·mL-1、15.16(13.78,16.66)μg·mL-1和13.33(11.03,15.13)μg·mL-1;不同浓度5-Fu及相等剂量的磁性层状复合氢氧化物、磁性层状复合氢氧化物-氟尿嘧啶、右旋糖酐-磁性层状复合氢氧化物-氟尿嘧啶对结肠癌SW480细胞分别干预24、48、72 h,细胞的增殖抑制率按磁性层状复合氢氧化物<<氟尿嘧啶<磁性层状复合氢氧化物-氟尿嘧啶<右旋糖酐-磁性层状复合氢氧化物-氟尿嘧啶升高,作用效果与剂量及时间有依赖关系;与原药相比,右旋糖酐-磁性层状复合氢氧化物-氟尿嘧啶对SW480细胞的增殖抑制率及早期凋亡率差异显著,能引起细胞核内染色质凝集、细胞解体。结论磁性层状复合氢氧化物细胞毒性低,能用作药物载体;磁性层状复合氢氧化物与氟尿嘧啶的2～3级超分子组装,具有相对原药更高的药物传输效率、能有效发挥抗肿瘤药物对SW480细胞的抑制与诱导凋亡作用。 Objective To investigate the inhibitory effect of dextran-magnetic layered double hydroxide-fluorouracil drug delivery system on the proliferation of colon cancer SW480 cells and the induction of apoptosis. Methods MTT assay was used to detect the inhibitory rates of dextran-magnetic layered double hydroxide-fluorouracil and all levels of supramolecules on the proliferation of colon cancer cells. Morphological changes were observed by light microscopy and Giemsa staining. Flow cytometry The apoptosis rate of SW480 cells induced by drug intervention was detected. DNA ladder was used to analyze the cleavage of apoptotic nuclei. Results The IC50 of magnetic layered composite hydroxide, magnetic layered composite hydroxide - fluorouracil and dextran - magnetic layered composite hydroxide - fluorouracil level 3 supramolecule on SW480 cells for 24 h were 44.83 (25.74 and 48.78) μg · mL-1, 15.16 (13.78,16.66) μg · mL-1 and 13.33 (11.03,15.13) μg · mL-1 respectively. Magnetic layered composite hydroxides with different concentrations of 5-Fu and equal doses, The composite hydroxides-fluorouracil, dextran-magnetic layered composite hydroxide-fluorouracil intervention on colon cancer SW480 cells were 24,48,72 h, the inhibition of cell proliferation by magnetic layered composite hydroxide << fluorouracil