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目的建立胃得安片白术内酯的检测方法,为其质量标准的改进提供依据。方法色谱柱:SHIMADZU C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相:甲醇-水(65∶35);流速:1.0 ml/min;检测波长220 nm,柱温:30℃。样品中白术内酯Ⅰ提取工艺为:甲醇超声处理30 min。结果白术内酯Ⅰ在2.4~38.4μg/ml范围内线性关系良好(r=0.9996、n=5),平均回收率为99.03%,RSD=0.37%。结论本方法简便可靠,重复性好,可用于该制剂的质量控制。
Objective To establish a method for the determination of Weidean Atractylodes, providing the basis for the improvement of its quality standard. Method Column: SHIMADZU C 18 column (250 mm × 4.6 mm, 5 μm); mobile phase: methanol-water (65:35); flow rate 1.0 ml / min; detection wavelength 220 nm; . Samples of Atractylol I extraction process: methanol ultrasonic treatment 30 min. Results Atractylol Ⅰ showed good linearity (r = 0.9996, n = 5) in the range of 2.4 ~ 38.4μg / ml with an average recovery of 99.03% and a RSD of 0.37%. Conclusion This method is simple and reliable, reproducible, and can be used for the quality control of the preparation.