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为了探讨栽培黄瓜(Cucumis sativusL.,2n=14)渐渗酸黄瓜(C.hystrix Chakr.,2n=24)遗传物质后的遗传变异情况,以12个黄瓜—酸黄瓜渐渗系及其受体亲本栽培黄瓜‘北京截头’为试验材料,对表现酸黄瓜主要特征的生物学性状进行调查分析和相关QTL检测。结果表明:各渐渗系的变异系数介于6.17%~46.26%,平均为20.81%,在多数性状上与受体亲本差异极显著,表现出酸黄瓜的遗传特性。通过t测验,以P≤0.001为阈值,在10个性状上共检测到87个QTL,平均每个性状9.67个QTL,每个渐渗系7.25个QTL,每个导入DNA片段0.8个QTL。上述结果证明控制相关性状的野生酸黄瓜遗传物质已渐渗进入栽培黄瓜,且使其遗传基础有所扩大。
In order to investigate the genetic variation of cucumber (Cucumis sativus L., 2n = 14) genetic material of C. hystrix Chak. (2n = 24), 12 Cucumber-Cucumber introgression lines and their receptors The cucumber ’Beijing’ truncated ’was used as the experimental material to investigate and analyze the biological traits that characterize the main characteristics of acid cucumber and the related QTLs. The results showed that the coefficient of variation of each of the introgression lines ranged from 6.17% to 46.26% with an average of 20.81%. The difference between the most introgressed lines and the recipient was very significant, showing the genetic characteristics of the cucumber. By means of t test, 87 QTLs were detected on 10 traits with P≤0.001 as threshold, 9.67 QTLs for each trait, 7.25 QTLs for each introgression line, and 0.8 QTLs for each introduced DNA fragment. The above results demonstrate that genetic control of wild acidified cucumber genetic material has gradually infiltrated into cucumber and its genetic basis has been expanded.