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目的 研究尿激酶型纤溶酶原激活剂 (uPA)和其特异性受体 (uPAR)在肝细胞癌 (HCC)的表达及其临床和病理意义。 方法 应用免疫组织化学和酶联免疫吸附法 (ELISA)检测uPA和uPAR在HCC和非癌组织中的表达 ,并与临床及病理指标相联系。 结果 HCC中uPA和uPAR的阳性表达率分别为 75 0 % (12 / 16 )和 6 8.8% (1/ 16 )。在进展性和侵袭性肿瘤与非进展性和非侵袭性肿瘤间uPA和uPAR阳性率无显著性差异 (P >0 .0 5 )。阳性染色主要位于癌细胞和基质细胞胞浆且主要位于癌侵犯的边缘。非癌组织仅见少量阳性染色细胞 ,对照组无特异性阳性染色。在ELISA中 ,癌与非癌组织uPA抗原水平分别为 (4 2 3± 0 5 7)ng/mg蛋白和 (1 2 6± 0 14)ng/mg蛋白 ,uPAR抗原水平分别为 (4 2 5± 0 2 1)ng/mg蛋白和 (3 15± 0 2 3)ng/mg蛋白 ,有显著性差异 (t=18.96 3,P =0 .0 0 0 ;t=13.6 93 ,P =0 .0 0 0 )。且进展性和侵袭性肿瘤中其抗原水平显著高于非进展性和非侵袭性肿瘤 (P <0 0 5 )。 结论 uPA和uPAR在肝细胞癌表达较高并与其进展和侵袭密切关联。
Objective To investigate the expression of urokinase-type plasminogen activator (uPA) and its specific receptor (uPAR) in hepatocellular carcinoma (HCC) and its clinical and pathological significance. Methods The expressions of uPA and uPAR in HCC and non-cancerous tissues were detected by immunohistochemistry and enzyme-linked immunosorbent assay (ELISA), and were correlated with clinical and pathological parameters. Results The positive rates of uPA and uPAR in HCC were 75 0% (12/16) and 6 8.8% (1/16), respectively. There was no significant difference in the positive rates of uPA and uPAR between progressive and invasive tumors and non-progressive and non-invasive tumors (P> 0.05). Positive staining is mainly located in the cytoplasm of cancer cells and stromal cells and is mainly located at the edge of cancer invasion. Non-cancerous tissue only a small amount of positive staining cells, the control group no specific positive staining. The levels of uPA antigen in cancer and non-cancerous tissue were (423 ± 0 57) ng / mg protein and (12 6 ± 0 14) ng / mg protein respectively in ELISA, the levels of uPAR antigen were (4 2 5 There were significant differences between the two groups (t = 18.96 3, P = 0.000; t = 13.693, P = 0). 0 0 0). The levels of antigens in aggressive and aggressive tumors were significantly higher than those in non-aggressive and non-invasive tumors (P <0.05). Conclusions uPA and uPAR are highly expressed in hepatocellular carcinoma and are closely associated with their progression and invasion.