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评价了燕麦镰刀菌GD-2毒素对野燕麦的除草活性潜力。毒素对野燕麦种子萌发抑制试验表明,当毒素浓度达到5 mg/mL时,对野燕麦种子的萌发抑制效果达77.54%,在相同浓度下,对野燕麦种子胚根的抑制效果高于对胚芽的抑制效果。在温室条件下,毒素处理野燕麦植株后,各个不同处理野燕麦株高和地上部鲜重明显减少,然而,各处理间没有明显差异。用石油醚、氯仿、乙酸乙酯和正丁醇依次萃取燕麦镰刀菌发酵滤液并获得粗提物,其中正丁醇浸提物(5 mg/mL)在离体叶片实验中效果优于其他有机溶剂粗提物。此外,在毒素对10种植物的敏感性实验中,其中稗草和藜表现对燕麦镰刀菌毒素敏感。从燕麦镰刀菌中分离出3个除草活性化合物,且具有相同的紫外吸收峰。当前结果表明燕麦镰刀菌产生的毒素具有开发成为防除野燕麦生物源除草剂的潜力。
The herbicide potential of oat Fusarium GD-2 to wild oats was evaluated. The toxin inhibition test on wild oat seed germination showed that when the toxin concentration reached 5 mg / mL, the inhibitory effect on wild oat seed germination was 77.54%. Under the same concentration, the inhibitory effect on wild oat seed radicle was higher than that on germ Inhibitory effect. Under the greenhouse conditions, after treatment with wild oats, the plant height and shoot fresh weight of wild oats treated with different treatments decreased significantly. However, there was no significant difference among treatments. The filtrate of Fusarium graminearum was extracted by petroleum ether, chloroform, ethyl acetate and n-butanol, and the crude extract was obtained. The n-butanol extract (5 mg / mL) was superior to other organic solvents Crude extract. In addition, in the sensitivity test of toxins to 10 species of plants, barnyardgrass and quinoa showed sensitivity to Fusarium venenatum toxin. Three herbicidally active compounds were isolated from Fusarium graminearum and had the same UV absorption peak. The current results show that the toxins produced by F. ovale have the potential to be developed as herbicide to control wild oat biogenic sources.