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目的探讨经尾静脉注射脂肪间充质干细胞是否对脑缺血再灌注损伤有一定的修复作用及相关机制。方法 45只SD大鼠随机均分为假手术组(sham),模型组(I/R)和治疗组(ADMSCs)。I/R和ADMSCs组大鼠采用线栓法大脑中动脉脑缺血再灌模型(MCAO)建立局灶性脑缺血再灌动物模型。缺血2h后再灌注,6ADMSCs组再灌注0和12h经尾静脉注射2.0×10个(0.5ml)脂肪间充质干细胞,I/R组注射等量的生理盐水。再灌注24h后处死大鼠并迅速取脑组织。通过TTC染色计算脑梗塞面积;TUNEL检测缺血部分脑组织中的细胞凋亡情况;Western blot检测缺血部分脑组织中i NOS和bcl-2/bax的表达水平。结果 ADMSCs组显著降低了脑梗塞体积,抑制神经细胞凋亡,减少Bax/bcl-2蛋白比,i NOS的表达水平明显下调。结论尾静脉注射ADMSCs修复脑I/R损伤可能与抑制神经细胞凋亡和i NOS表达相关。
Objective To investigate whether intratracheal instillation of adipose-derived mesenchymal stem cells can repair cerebral ischemia-reperfusion injury and its underlying mechanisms. Methods 45 Sprague - Dawley rats were randomly divided into sham group, model group (I / R) and treatment group (ADMSCs). The rats in I / R and ADMSCs group were subjected to focal cerebral ischemia-reperfusion (IRI) model by middle cerebral artery occlusion (MCAO). After ischemia 2h, reperfusion was performed. In the 6ADMSCs group, 2.0 × 10 (0.5ml) adipose tissue-derived mesenchymal stem cells were injected via tail vein at 0 and 12h after reperfusion. The rats in I / R group were injected with the same amount of saline. After 24 hours of reperfusion, the rats were killed and the brain tissue was taken quickly. The area of cerebral infarction was calculated by TTC staining. The apoptosis of ischemic brain tissue was detected by TUNEL. The expression of iNOS and bcl-2 / bax in ischemic brain tissue was detected by Western blot. Results The ADMSCs group significantly reduced the volume of cerebral infarction, inhibited the apoptosis of nerve cells and decreased the ratio of Bax / bcl-2 protein. The expression of iNOS was significantly down-regulated. Conclusion The tail vein injection of ADMSCs to repair brain I / R injury may be related to inhibition of neuronal apoptosis and iNOS expression.