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采用软模板法合成出介孔酚醛树脂球,通过部分碳化法获得有序介孔纳米球,考察了水浴反应时溶液的p H及模板剂对产物形态的影响,当反应溶液p H=8.0及模板剂为F127时,合成产物为规则纳米球状,球体大小均一且介孔明显。通过扫描电镜(SEM)及透射电镜(TEM)观察到产物表面有明显有序介孔存在,红外分析证明了其表面存在诸多活性基团。介孔碳纳米球成功负载阿霉素,载药率为38.2%,96 h后的药物释放率为58.5%。通过介孔碳材料与人成纤维细胞共培养以考察材料的生物相容性,利用吖啶橙(AO)染色观察细胞状态,采用MTT(3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四氮唑噻唑蓝)法进行细胞增殖定量检测,结果表明:与介孔碳球共培养的细胞形态正常,结构完整,伪足清晰可辩,生长状况良好;共培养7 d后细胞存活率保持在80%以上,证明介孔碳纳米球有良好的生物相容性。
Mesoporous phenolic resin spheres were synthesized by soft template method, and ordered mesoporous nanospheres were obtained by partial carbonation. The effect of p H and template on the product morphology was investigated. When the reaction solution p H = 8.0 and When the template agent is F127, the synthesized product is a regular nanosphere, the sphere size is uniform and the mesopores are obvious. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the surface of the product was clearly ordered mesopores. Infrared analysis showed that there were many active groups on the surface. The mesoporous carbon nanospheres were loaded with doxorubicin successfully, the drug loading rate was 38.2%, and the drug release rate after 96 h was 58.5%. The mesoporous carbon materials were co-cultured with human fibroblasts to investigate the biocompatibility of the materials. The cell morphology was observed by acridine orange (AO) staining. MTT (3- (4,5-dimethyl-2-thiazole ) -2,5-diphenyltetrazolium bromide tetrazolium thiazolyl blue) method for quantitative detection of cell proliferation, the results showed that: mesocarbon co-culture of cells with normal morphology, structural integrity, pseudopodia clear debate, the growth status Good; 7 days after co-cultured cell survival rate remained above 80%, to prove that the mesoporous carbon nanospheres have good biocompatibility.