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利用超高效液相色谱-飞行时间质谱联用技术研究不同配伍比例的丹参藜芦的化学指纹图谱,并分析毒性生物碱在不同比例配伍混煎前后溶出量的变化.同时利用小鼠的急性毒性实验来考察配伍后的毒性变化规律.结果显示在正离子模式下,各配伍组(A～H)样本之间的差异能得到明显区分,并结合Loadings图谱和数据库确定了配伍化学指纹图谱中的17种藜芦生物碱毒性成分,当固定藜芦用量为LD50且藜芦比例大于丹参时,藜芦定、伪介芬胺等生物碱溶出高于或与藜芦单煎液相当,但随着丹参比例增加,上述生物碱溶出呈逐渐下降趋势,当丹参比例大于藜芦时,生物碱溶出低于藜芦单煎液.不同比例丹参藜芦配伍急性毒性实验毒性变化规律与上述生物碱含量的变化趋势吻合,表明上述毒性生物碱可能是藜芦与丹参特定比例配伍后毒性增强主要化学标志物.
The chemical fingerprints of Radix Salviae Miltiorrhizae with different compatibility ratios were studied by ultra performance liquid chromatography-coupled with time-of-flight mass spectrometry, and the changes of the dissolution of toxic alkaloids before and after different proportions of mixed decoction were analyzed. At the same time, the acute toxicity The results showed that in the positive ion mode, the difference between the samples of different compatibility groups (A ~ H) could be clearly distinguished, and the combination of Loadings pattern and database was used to confirm the compatibility of chemical fingerprint 17 kinds of veratridine alkaloids toxic components, when the amount of fixed veratrum LD50 and veratrum is greater than the proportion of Salvia, veratridine, pseudo-acetazepril and other alkaloids eluted more or less than the same as with the veratridine decoction, but with the When the proportion of Salvia miltiorrhiza is bigger than that of Veratrum, the dissolution of alkaloids is lower than that of Tripterygium reifu decoction. The change rule of the acute toxicities of Radix Salviae Miltiorrhizae in different proportions is consistent with the above-mentioned alkaloid content The trend of change is consistent, indicating that the above toxic alkaloids may be the main chemical marker of toxicity enhancement after the compatibility of veratridine and Salvia miltiorrhiza.