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本文以双霉协联糖化技术处理粉渣中纤维素,并利用单因素和正交试验考察菌种混合比例、接种量、协联糖化温度与协联糖化时间对纤维素糖化率的影响,结果表明:双霉协联糖化的最优条件为:少孢根霉与里氏木霉混合比值为1.5,接种量为1.8×108个孢子/mL,协联糖化温度为34℃,协联糖化时间为72h,在此工艺条件下纤维素糖化率高达82.3%。纤维素酶活力达到645.5～664U/mL,与传统单菌种发酵的糖化技术相比,提高了160～280U/mL。
In this paper, the double mold co-saccharification technology to deal with the residue of cellulose powder, and the use of single factor and orthogonal test to investigate the mixing ratio of bacteria, inoculum size, co-concordation of saccharification temperature and time of saccharification on cellulose saccharification rate, the results The results showed that the optimal conditions for the co-saccharification of mold were as follows: the mixture ratio of Rhizopus sp. And Trichoderma reesei was 1.5, the inoculum size was 1.8 × 108 spores / mL, the co-saccharification temperature was 34 ℃, 72h, under the conditions of the cellulose saccharification rate of up to 82.3%. Cellulase activity reached 645.5 ~ 664U / mL, compared with the traditional single-species fermentation saccharification technology, increased by 160 ~ 280U / mL.