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运用PCR、PCR -SSCP、PCR产物直接测序等方法对 6 1例少精子症及无精子症患者的精蛋白基因 5′端及 3′端核基质结合区 (Matrixattachmentregion ,MAR)进行了突变筛查。结果发现 2例少精子患者在 5′端核基质结合区存在PCR -SSCP带型变异 ,经测序分析发现其碱基序列存在一杂合性改变
A total of 61 cases of oligospermia and azoospermia were enrolled in this study. Mutations were detected in the 5 ’and 3’ end of the protamine gene by PCR, PCR-SSCP and PCR products. . The results showed that two cases of oligospermia patients in the 5 ’end nuclear stromal binding zone PCR-SSCP band-type variation found by sequencing analysis of the base sequence of a heterozygous changes